Recombinant:Article Title: Antibody specifically binding to IL-17A, encoding nucleic acid, and method of using the antibody
Article Snippet: .. Recombinant human IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, IL-17F, IL-1, IL-2, IL-6, IL-8, IL-21, IL-22, IL-23, IFN-g and TNFα (purchased from Sino Biological Inc. or R&D systems) were coated on a 96-well high-absorbing ELISA plate with a carbonate buffer solution with pH 9.6, the coating concentration was 1 μg/mL, the coating amount was 100 μL per well, and the coating was carried at 4° C. out overnight. ..
Article Title: Anti-IL-17 antibody/TNFR ECD fusion protein and use thereof
Article Snippet: The anti-IgG antibody captured the BY19.3 protein to produce 1600RU; Recombinant human TNF-α (Sino Biological Inc., catalog number: 10602-HNAE) was diluted to 80 nM, injected at a flow rate of 20 μL/min for 2 minutes. .. BY19.3 protein captured recombinant human TNF-α protein, produced 361RU, and reached saturation; then recombinant human IL-17A (Sino Biological Inc., catalog number: 12047-H07Y) was diluted to 80 nM, injected at a flow rate of 20 μL/min for 2 minutes, the chip having bound to TNF-α protein and reached saturation state can further capture recombinant human IL-17A protein, producing 290RU and reaching saturation. ..
Article Title: Antibody specifically binding to IL-17A, encoding nucleic acid, and method of using the antibody
Article Snippet: .. Recombinant human IL-17A, monkey IL-17A, rat IL-17A and mouse IL-17A (all purchased from Sino Biological Inc.), were coated on a 96-well high-absorbing ELISA plate with a carbonate buffer solution with pH 9.6, the coating concentration was 1 g/mL, the coating amount was 100 μL per well, and the coating was carried out at 4° C. overnight. ..
Article Title: An IGFBP7 hi endothelial cell subset drives T cell extravasation in psoriasis via endothelial glycocalyx degradation
Article Snippet: .. Second, to identify the upstream cytokines leading to upregulated IGFBP7 expression, recombinant human IL-17A (Sino Biological, cat. no. CT047-HNAE), IL-22 (Sino Biological, cat. no. 13059-HNAE), IL-25 (Sino Biological, cat. no. 10096-H01H), IFN-γ (Sino Biological, cat. no. 11725-HNAS), TNF-⍺ (Sino Biological, cat. no. 10602-HNAE), and VEGF-A (Sino Biological, cat. no. 29714-HNAH) at a series of concentrations (20, 50, and 100 ng/mL) were added to the culture medium of HMEC-1 cells. ..
Article Title: Antibody specifically binding to IL-17A, encoding nucleic acid, and method of using the antibody
Article Snippet: .. Recombinant human IL-17A (purchased from Sino Biological Inc.) was coated on a 96-well high-absorbing ELISA plate with a carbonate buffer solution with pH 9.6, the coating concentration was 1 μg/mL, the coating amount was 100 μL per well, and the coating was carried at 4° C. out overnight. ..
Article Title: Anti-IL-17 antibody/TNFR ECD fusion protein and use thereof
Article Snippet: Centrifuge at 1500 rpm for 5 min, discard the supernatant; then resuspend the cells in 10 ml DMEM/F12 medium (purchased from Hyclone); count cell density; plant 96-well plate at 100 μl/well (2×104 cells/well); place in 37° C., 5% CO2 incubator for overnight cultivation. .. Recombinant human TNF-α (Sino Biological Inc., catalog number: 10602-HNAE) and recombinant human IL-17A (Sino Biological Inc., catalog number: 12047-H07Y) were freshly prepared on the day of the experiment, respectively. ..
Article Title: Antibody specifically binding to IL-17A, encoding nucleic acid, and method of using the antibody
Article Snippet: .. Recombinant human IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, IL-17F, IL-1, IL-2, IL-6, IL-8, IL-21, IL-22, IL-23, IFN-g and TNFα (purchased from Sino Biological Inc. or R&D systems) were coated on a 96-well high-absorbing ELISA plate with a carbonate buffer solution with pH 9.6, the coating concentration was 1 μg/mL, the coating amount was 100 μL per well, and the coating was carried ax 4° C. out overnight. ..
Article Title: Antibody targeting interleukin 17A and preparation method and application thereof
Article Snippet: .. Experiment Material: Recombinant Human IL-17A, Sino Biological, 12047-HNAS Recombinant human IL-17B, R&D, 8129-IL Recombinant human IL-17D, Sino Biologica, 10076-H08S Recombinant human IL-17E, ABGENT, PBV10154r Recombinant human IL-17F, Sino Biological, 11855-H07H-5 Recombinant human IL-17A/F, Sino Biological, CT047-H08H-20 Experiment Method: The method was the same as that in Examples 1. ..
Enzyme-linked Immunosorbent Assay:Article Title: Antibody specifically binding to IL-17A, encoding nucleic acid, and method of using the antibody
Article Snippet: .. Recombinant human IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, IL-17F, IL-1, IL-2, IL-6, IL-8, IL-21, IL-22, IL-23, IFN-g and TNFα (purchased from Sino Biological Inc. or R&D systems) were coated on a 96-well high-absorbing ELISA plate with a carbonate buffer solution with pH 9.6, the coating concentration was 1 μg/mL, the coating amount was 100 μL per well, and the coating was carried at 4° C. out overnight. ..
Article Title: Antibody specifically binding to IL-17A, encoding nucleic acid, and method of using the antibody
Article Snippet: .. Recombinant human IL-17A, monkey IL-17A, rat IL-17A and mouse IL-17A (all purchased from Sino Biological Inc.), were coated on a 96-well high-absorbing ELISA plate with a carbonate buffer solution with pH 9.6, the coating concentration was 1 g/mL, the coating amount was 100 μL per well, and the coating was carried out at 4° C. overnight. ..
Article Title: Antibody specifically binding to IL-17A, encoding nucleic acid, and method of using the antibody
Article Snippet: .. Recombinant human IL-17A (purchased from Sino Biological Inc.) was coated on a 96-well high-absorbing ELISA plate with a carbonate buffer solution with pH 9.6, the coating concentration was 1 μg/mL, the coating amount was 100 μL per well, and the coating was carried at 4° C. out overnight. ..
Article Title: Antibody specifically binding to IL-17A, encoding nucleic acid, and method of using the antibody
Article Snippet: .. Recombinant human IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, IL-17F, IL-1, IL-2, IL-6, IL-8, IL-21, IL-22, IL-23, IFN-g and TNFα (purchased from Sino Biological Inc. or R&D systems) were coated on a 96-well high-absorbing ELISA plate with a carbonate buffer solution with pH 9.6, the coating concentration was 1 μg/mL, the coating amount was 100 μL per well, and the coating was carried ax 4° C. out overnight. ..
Concentration Assay:Article Title: Antibody specifically binding to IL-17A, encoding nucleic acid, and method of using the antibody
Article Snippet: .. Recombinant human IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, IL-17F, IL-1, IL-2, IL-6, IL-8, IL-21, IL-22, IL-23, IFN-g and TNFα (purchased from Sino Biological Inc. or R&D systems) were coated on a 96-well high-absorbing ELISA plate with a carbonate buffer solution with pH 9.6, the coating concentration was 1 μg/mL, the coating amount was 100 μL per well, and the coating was carried at 4° C. out overnight. ..
Article Title: Antibody specifically binding to IL-17A, encoding nucleic acid, and method of using the antibody
Article Snippet: .. Recombinant human IL-17A, monkey IL-17A, rat IL-17A and mouse IL-17A (all purchased from Sino Biological Inc.), were coated on a 96-well high-absorbing ELISA plate with a carbonate buffer solution with pH 9.6, the coating concentration was 1 g/mL, the coating amount was 100 μL per well, and the coating was carried out at 4° C. overnight. ..
Article Title: Antibody specifically binding to IL-17A, encoding nucleic acid, and method of using the antibody
Article Snippet: .. Recombinant human IL-17A (purchased from Sino Biological Inc.) was coated on a 96-well high-absorbing ELISA plate with a carbonate buffer solution with pH 9.6, the coating concentration was 1 μg/mL, the coating amount was 100 μL per well, and the coating was carried at 4° C. out overnight. ..
Article Title: Antibody specifically binding to IL-17A, encoding nucleic acid, and method of using the antibody
Article Snippet: .. Recombinant human IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, IL-17F, IL-1, IL-2, IL-6, IL-8, IL-21, IL-22, IL-23, IFN-g and TNFα (purchased from Sino Biological Inc. or R&D systems) were coated on a 96-well high-absorbing ELISA plate with a carbonate buffer solution with pH 9.6, the coating concentration was 1 μg/mL, the coating amount was 100 μL per well, and the coating was carried ax 4° C. out overnight. ..
Produced:Article Title: Anti-IL-17 antibody/TNFR ECD fusion protein and use thereof
Article Snippet: The anti-IgG antibody captured the BY19.3 protein to produce 1600RU; Recombinant human TNF-α (Sino Biological Inc., catalog number: 10602-HNAE) was diluted to 80 nM, injected at a flow rate of 20 μL/min for 2 minutes. .. BY19.3 protein captured recombinant human TNF-α protein, produced 361RU, and reached saturation; then recombinant human IL-17A (Sino Biological Inc., catalog number: 12047-H07Y) was diluted to 80 nM, injected at a flow rate of 20 μL/min for 2 minutes, the chip having bound to TNF-α protein and reached saturation state can further capture recombinant human IL-17A protein, producing 290RU and reaching saturation. ..
Injection:Article Title: Anti-IL-17 antibody/TNFR ECD fusion protein and use thereof
Article Snippet: The anti-IgG antibody captured the BY19.3 protein to produce 1600RU; Recombinant human TNF-α (Sino Biological Inc., catalog number: 10602-HNAE) was diluted to 80 nM, injected at a flow rate of 20 μL/min for 2 minutes. .. BY19.3 protein captured recombinant human TNF-α protein, produced 361RU, and reached saturation; then recombinant human IL-17A (Sino Biological Inc., catalog number: 12047-H07Y) was diluted to 80 nM, injected at a flow rate of 20 μL/min for 2 minutes, the chip having bound to TNF-α protein and reached saturation state can further capture recombinant human IL-17A protein, producing 290RU and reaching saturation. ..
Expressing:Article Title: An IGFBP7 hi endothelial cell subset drives T cell extravasation in psoriasis via endothelial glycocalyx degradation
Article Snippet: .. Second, to identify the upstream cytokines leading to upregulated IGFBP7 expression, recombinant human IL-17A (Sino Biological, cat. no. CT047-HNAE), IL-22 (Sino Biological, cat. no. 13059-HNAE), IL-25 (Sino Biological, cat. no. 10096-H01H), IFN-γ (Sino Biological, cat. no. 11725-HNAS), TNF-⍺ (Sino Biological, cat. no. 10602-HNAE), and VEGF-A (Sino Biological, cat. no. 29714-HNAH) at a series of concentrations (20, 50, and 100 ng/mL) were added to the culture medium of HMEC-1 cells. ..
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